Volume 7, Issue 2, April 2019, Page: 28-33
Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment
Karim Mohamed Beltagy, Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Manal Moustafa Abdel-Rahman, Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Hanaa Mahdy Abouzied, Agronomy Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Samia Ahmed Madkour, Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Received: Mar. 4, 2019;       Accepted: Mar. 19, 2019;       Published: May 7, 2019
DOI: 10.11648/j.bio.20190702.11      View  173      Downloads  20
Abstract
The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.
Keywords
Maize, Biotic Stress, Anthranilate Synthase, Gus Assay, Particle Bombardment
To cite this article
Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour, Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment, American Journal of Bioscience and Bioengineering. Vol. 7, No. 2, 2019, pp. 28-33. doi: 10.11648/j.bio.20190702.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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