Modified Atkins Diet Delayed the Onset of Epileptogenesis, Improved Motor Coordination and Enhanced Learning Memory in Mice
Maryam Adenike Salaudeen,
Mohammed Garba Magaji,
Abdulkadir Umar Zezi
Issue:
Volume 11, Issue 1, February 2023
Pages:
1-6
Received:
29 December 2022
Accepted:
25 January 2023
Published:
4 March 2023
Abstract: Modified Atkins diet is a high fat, moderate protein and carbohydrate diet that has long been used to manage intractable epilepsy in developed countries. However, the use of this diet for same condition is uncommon in most low- and middle-income countries including Nigeria. In a previous research, we formulated modified Atkins diet using cheap and locally-available food materials in Nigeria. This research is thus, aimed at evaluating the effect of the formulated modified Atkins diet on seizure plasticity using the PTZ-induced kindling model of seizure. We also investigated the effect of sub-chronic use (60 days) of the diet on motor coordination using positional sense test and on visual-spatial reference and learning memory using Morris-water maze. The result revealed that the formulated modified Atkins diet significantly (p<0.05) increased seizure plasticity by reducing the severity of seizure as well as delaying the onset of tonic-clonic seizure and death. The diet also enhanced motor coordination, and improved learning memory, but not reference memory in mice. We therefore concluded that the formulated modified Atkins diet may be of benefit in the management of intractable/treatment-resistant epilepsy, especially in patients who are already suffering from amnesia and/or postural instability since the diet was able to offer protection against seizure-induced memory loss and motor-incoordination in mice.
Abstract: Modified Atkins diet is a high fat, moderate protein and carbohydrate diet that has long been used to manage intractable epilepsy in developed countries. However, the use of this diet for same condition is uncommon in most low- and middle-income countries including Nigeria. In a previous research, we formulated modified Atkins diet using cheap and ...
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Rapid Evaluation of Anti-inflammatory Effect of Geniopicroside
Zijia Liu,
Yidong Tu,
Tianbi Duan,
Zhi Lv,
Ruixue Yin,
Hongbo Zhang
Issue:
Volume 11, Issue 1, February 2023
Pages:
7-13
Received:
9 May 2023
Accepted:
26 May 2023
Published:
29 May 2023
Abstract: Skin acts as a barrier and is an important part of body immune system. External physical, biochemical and other stimuli might cause skin inflammation. Drugs and cosmetics have been developed for skin inflammation treatment. At present, the tests of skin inflammation are mainly conducted on animal and 2D cell models. The former might bring problems such as ethics and authenticity, while the latter cannot fully represent the complex micro-environment of the human body. Microfluidic based Organ-on- a- chips technology provides a new method for drug and cosmetic ingredient screening. Skin-on-a-chip (SOC) has been designed for constructing in vitro skin models. In this paper, a SOC was developed to culture skin-like models in vitro. We tested the differentiation of SOC cultured skin model, and the results showed that its stratum corneum was well differentiated. It indicates that the skin tissue cultured by the SOC bears some similarities to human skin, which can be used for subsequent drug testing. We tested the anti-inflammatory effect of gentiopicroside and compared with dexamethasone. The results showed that 5μg/ml~50μg/ml of gentiopicroside had similar anti-inflammatory effect to 1μM of dexamethasone.
Abstract: Skin acts as a barrier and is an important part of body immune system. External physical, biochemical and other stimuli might cause skin inflammation. Drugs and cosmetics have been developed for skin inflammation treatment. At present, the tests of skin inflammation are mainly conducted on animal and 2D cell models. The former might bring problems ...
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